Conveners
Wounds & healing
- Hayden Tronnolone (University of Adelaide)
In order to explore the role of initial geometry in wound closure, we developed a new two-dimensional wound healing assay which we refer to as a sticker assay. Stickers are produced and attached to a tissue culture plate before cells are seeded into the centre of a dish. The stickers are then removed to leave a wound in the dish, which is an area with no cells. We performed a number of...
A leg wound which does not heal because of problems with the veins in the leg is called a venous leg ulcer (VLU). Chronic VLUs are the most common chronic wounds in western countries and their treatment is both costly and time consuming. In this work, we present a mathematical model of the healing of a VLU which incorporates the key biological features of this wound type. We have modelled the...
Scratch assays are routinely used to study the collective spreading of cell populations. In general, the rate at which a population of cells spreads is driven by the combined effects of cell migration and proliferation. To examine the effects of cell migration separately from the effects of cell proliferation, scratch assays are often performed after treating the cells with a drug that...
Scratch assays are standard in vitro experimental methods for studying cell migration. In these experiments, a scratch is made on a cell monolayer and imaging of the recolonisation of the scratched region is performed to quantify cell migration rates. Typically, scratch assays are modelled by continuum reaction diffusion equations depicting cell migration by diffusion and carrying...